Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
The Human NT-proBNP (N-Terminal pro-Brain Natriuretic Peptide) CLIA Kit is specifically designed for the accurate measurement of NT-proBNP levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.NT-proBNP is a biomarker that plays a crucial role in heart function and cardiovascular diseases. This peptide is released from the heart in response to increased cardiac stress and is used as a diagnostic tool for conditions such as heart failure, myocardial infarction, and hypertension. By accurately measuring NT-proBNP levels, researchers can gain valuable insights into cardiac health and disease progression, leading to advancements in diagnostic and therapeutic strategies. Order the Human NT-proBNP CLIA Kit today to enhance your research efforts in cardiovascular medicine.
Product Name:
Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
SKU:
HUES00541
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
