Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
The Rat NT-proBNP (N-terminal pro-brain natriuretic peptide) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of NT-proBNP levels in rat samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.NT-proBNP is a biomarker associated with cardiac dysfunction and heart failure, making it a valuable marker for monitoring and diagnosing cardiovascular diseases in animal models. Measuring NT-proBNP levels can provide insights into cardiac function and help researchers better understand the mechanisms underlying heart failure.With its high sensitivity and specificity, the Rat NT-proBNP CLIA Kit from Assay Genie is a valuable tool for researchers studying cardiac biomarkers and exploring potential therapies for cardiovascular diseases in rat models.
Product Name:
Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
SKU:
RTES00412
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.