Human RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit
The Human RBP4 (Retinol Binding Protein 4) Plasma CLIA Kit is a cutting-edge tool for the quantitative detection of RBP4 levels in human plasma samples. This kit offers exceptional sensitivity and specificity, providing researchers with accurate and reliable results for a variety of research applications.RBP4 is a key protein known for its role in transporting retinol and regulating insulin sensitivity. Dysregulation of RBP4 levels has been associated with various metabolic disorders, making it a valuable biomarker for studying conditions such as obesity, diabetes, and cardiovascular diseases.
With its high precision and easy-to-use format, the Human RBP4 Plasma CLIA Kit is an essential tool for researchers looking to explore the role of RBP4 in health and disease. Order yours today from Assaygenie to enhance your research efforts and discover new insights in the field of biomarker detection.
Product Name:
Human RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit
SKU:
HUES00897
Target:
Human RBP4 (Retinol Binding Protein 4, Plasma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RBP4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RBP4. You can calculate the concentration of Human RBP4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
90-104
94-110
Average (%)
99
95
102
1:4
Range (%)
94-110
94-107
92-105
Average (%)
100
100
97
1:8
Range (%)
102-119
95-107
84-97
Average (%)
109
102
90
1:16
Range (%)
102-118
98-113
93-109
Average (%)
108
105
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-113
108
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
98-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.72
7.42
0.88
2.55
6.87
Standard deviation
0.1
0.28
0.54
0.11
0.19
0.43
C V (%)
10.64
10.29
7.28
12.5
7.45
6.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RBP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RBP4 in samples. No significant cross-reactivity or interference between Human RBP4 and analogues was observed.
