Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit
The Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit is specifically designed for the accurate quantification of VEGF165 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential tool for various research applications.VEGF165 is a critical growth factor that plays a key role in angiogenesis, the process of forming new blood vessels. It is involved in various physiological and pathological processes, including wound healing, tumor growth, and cardiovascular diseases. By measuring VEGF165 levels, researchers can gain valuable insights into these conditions and potential therapeutic interventions.Overall, the Human VEGF165 ELISA Kit is a valuable resource for studying the role of VEGF165 in various biological processes and diseases, ultimately contributing to the advancement of scientific research and clinical development.
Product Name:
Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit
SKU:
HUES03060
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
