The [KO Validated] CD168/RHAMM Monoclonal Antibody (CAB11666) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] CD168/RHAMM Monoclonal Antibody
SKU:
CAB11666
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC0667
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:100 - 1:500
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD168, IHABP, RHAMM, CD168/RHAMM
Positive Sample:
K-562
Cellular Localization:
Cell Surface, Cytoplasm.
Calculated MW:
84kDa
Observed MW:
84kDa
The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method
Affinity purification
Gene ID
3161
RRID
AB_2861623
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using CD168/RHAMM Rabbit mAb (CAB11666) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using CD168/RHAMM Rabbit mAb (CAB11666) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
