Anti-M6PR Antibody (CAB19907)[KO Validated]

SKU:
CAB19907
€129 - €349

Description

Technical Manual

Anti-M6PR Antibody [KO Validated]

Product Name:Anti-M6PR Antibody [KO Validated]
Product SKU:CAB19907
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X.

Immunogen:Recombinant protein of human M6PR.
Sequence:

Email for sequence

Gene ID:4074
Swiss Prot:P20645
Synonyms:M6PR; CD-M6PR; CD-MPR; MPR 46; MPR-46; MPR46; SMPR
Calculated MW:30kDa
Observed MW:31KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry
Positive Samples:293T
Cellular Location:Lysosome membrane, Single-pass type I membrane protein

Product Images

Anti-M6PR Antibody [KO Validated] (CAB19907)

Western blot analysis of extracts from normal (control) and M6PR knockout (KO) 293T cells, using M6PR antibody (CAB19907) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Uniprot Information

UniProt Protein Function:M6PR: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: endosome; integral to plasma membrane; membrane; perinuclear region of cytoplasm; trans-Golgi network; trans-Golgi network membrane; transport vesicle

Molecular Function:transmembrane receptor activity

Biological Process: endosome to lysosome transport; receptor-mediated endocytosis

NCBI Summary:This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, May 2011]
UniProt Code:P20645
NCBI GenInfo Identifier:127293
NCBI Gene ID:4074
NCBI Accession:P20645.1
UniProt Secondary Accession:P20645,A8K528, D3DUV5,
UniProt Related Accession:P20645
Molecular Weight:30,993 Da
NCBI Full Name:Cation-dependent mannose-6-phosphate receptor
NCBI Synonym Full Names:mannose-6-phosphate receptor, cation dependent
NCBI Official Symbol:M6PR
NCBI Official Synonym Symbols:SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
NCBI Protein Information:cation-dependent mannose-6-phosphate receptor
UniProt Protein Name:Cation-dependent mannose-6-phosphate receptor
UniProt Synonym Protein Names:46 kDa mannose 6-phosphate receptor; MPR 46
Protein Family:Cation-dependent mannose-6-phosphate receptor
UniProt Gene Name:M6PR
UniProt Entry Name:MPRD_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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