The LTD4 (Leukotriene D4) ELISA Kit is designed for the quantitative detection of Leukotriene D4 (LTD4) levels in various biological samples. LTD4 is a potent inflammatory mediator involved in a wide range of cellular activities, including bronchoconstriction, vascular permeability and leukocyte adhesion. It significantly influences the body's immune response by causing smooth muscle contractions and has been implicated in various pathological conditions like asthma, allergic rhinitis, and cardiovascular diseases. Accurate measurement of LTD4 is crucial for understanding its involvement in disease pathogenesis and for the development of targeted therapeutic strategies. Assay Genie's LTD4 (Leukotriene D4) ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an ideal choice for both research and clinical applications. Trust Assay Genie's LTD4 ELISA Kit for accurate and dependable quantification of this key biomarker in your investigations.
Product Name:
LTD4 (Leukotriene D4) ELISA Kit
SKU:
AEES00003
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
