The MARCH2 Antibody (PACO4525) is a valuable tool for researchers studying MARCH2, a cell surface molecule involved in immune regulation. This polyclonal antibody, produced in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By binding specifically to the MARCH2 protein, this antibody allows for accurate detection and analysis in various cell types, making it an excellent choice for studies in immunology and cancer research.MARCH2, also known as membrane-associated RING-CH 2, plays a crucial role in immune response regulation by controlling the expression of cell surface molecules. Its involvement in immune system function makes it a promising target for research on diseases like cancer, autoimmune disorders, and inflammatory conditions.
Understanding the role of MARCH2 is essential for developing innovative therapies that manipulate immune function in these disease contexts.Overall, the MARCH2 Antibody (PACO4525) is a reliable tool for investigating the biological functions of MARCH2 and its potential implications in various disease states. Whether studying immune modulation, tumor immunity, or therapeutic targeting, this antibody provides researchers with a valuable resource for advancing their understanding of immune regulation mechanisms.
Antibody Name:
MARCH2 Antibody
Antibody SKU:
PACO04525
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC, IF
Recommended Dilutions:
IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:
Human
Immunogen:
synthesized peptide derived from the C-terminal region of human 40604.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
MARCH2; RNF172; HSPC240; E3 ubiquitin-protein ligase MARCH2; Membrane-associated RING finger protein 2; Membrane-associated RING-CH protein II; MARCH-II; RING finger protein 172
UniProt Protein Function:
MARCH2: E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligaseChromosomal Location of Human Ortholog: 19p13.2Cellular Component: cytoplasmic vesicle; endoplasmic reticulumMolecular Function: protein binding; ubiquitin-protein ligase activityBiological Process: protein ubiquitination
UniProt Protein Details:
NCBI Summary:
MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM, Mar 2010]
