The Mouse D-LDH (D-Lactate Dehydrogenase) ELISA Kit is specially designed for the precise measurement of D-LDH levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.D-Lactate Dehydrogenase plays a vital role in the conversion of D-lactate to pyruvate in the glycolytic pathway. Abnormal levels of D-LDH have been associated with various metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential treatments. With a user-friendly protocol and quick assay time, the Mouse D-LDH ELISA Kit provides researchers with a reliable tool for investigating D-LDH levels in biological samples. Whether studying metabolic diseases, tissue ischemia, or microbial infections, this kit is essential for precise and efficient analysis.
Product Name:
Mouse D-LDH (D-Lactate Dehydrogenase) ELISA Kit
SKU:
MOES00965
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
2.81 mIU/mL
Detection range:
4.69-300 mIU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
