The Human D-Lactate Dehydrogenase (D-LDH) ELISA Kit from Assay Genie is an innovative tool for the accurate measurement of D-LDH levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.D-Lactate Dehydrogenase is an important enzyme involved in metabolic processes, particularly in the conversion of lactate to pyruvate. Dysregulation of D-LDH levels has been associated with various pathological conditions such as liver disease, muscle injury, and metabolic disorders. This ELISA kit allows researchers to study the role of D-LDH in these conditions and explore potential therapeutic interventions.With its user-friendly protocol and quick assay time, the Human D-Lactate Dehydrogenase ELISA Kit from Assay Genie is a valuable tool for researchers interested in studying the functions and implications of D-LDH in human health and disease.
Product Name:
Human D-LDH (D-Lactate Dehydrogenase) ELISA Kit
SKU:
HUES01994
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
