The Mouse INHB (Inhibin-B) ELISA Kit is specifically designed for the precise measurement of inhibin-B levels in mouse serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.Inhibin-B is a key protein that plays a vital role in regulating the reproductive system, particularly in the synthesis and secretion of follicle-stimulating hormone (FSH) from the pituitary gland. Abnormal levels of inhibin-B have been linked to disorders such as infertility, polycystic ovarian syndrome (PCOS), and ovarian cancer, making it a valuable biomarker for investigating these conditions and developing potential treatments.With its advanced technology and reliable performance, the Mouse INHB (Inhibin-B) ELISA Kit is an essential tool for researchers studying reproductive biology and related disorders in mouse models.
Product Name:
Mouse INHB (Inhibin B) ELISA Kit
SKU:
MOES01195
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.