Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
The Mouse MIP-1 alpha (Macrophage Inflammatory Protein-1 alpha) ELISA Kit is a reliable and accurate tool for detecting levels of MIP-1 alpha in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it perfect for a variety of research applications.MIP-1 alpha is a key chemokine involved in the inflammatory response, attracting and activating immune cells such as macrophages and T cells. It plays a crucial role in various biological processes, including immune response regulation and inflammation resolution. Studying MIP-1 alpha levels can provide essential insights into inflammatory diseases, immune disorders, and other pathological conditions, offering valuable information for potential therapeutic developments.Overall, the Mouse MIP-1 alpha (Macrophage Inflammatory Protein-1 alpha) ELISA Kit is a valuable tool for researchers seeking to investigate the role of MIP-1 alpha in various biological processes and disease states, ultimately advancing our understanding of immune response mechanisms and potential treatment options.
Product Name:
Mouse MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
SKU:
MOES00628
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
