Rat MIP-1alpha (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
The Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit is a specialized assay tailored for the quantitative measurement of MIP-1α levels in rat biological samples. MIP-1α is a key chemokine involved in orchestrating inflammatory responses, particularly in recruitment and activation of immune cells, such as monocytes and lymphocytes, in various physiological and pathological conditions. Our ELISA kit offers a precise and reliable method for detecting and quantifying MIP-1α levels, enabling researchers to gain insights into the immune response modulation, inflammation processes, and disease pathogenesis. With exceptional sensitivity and specificity, the Assay Genie's IL-1 ELISA Kit ensures accurate and reproducible results, setting a gold standard for research in immunology, inflammation, and related fields. Trust in our kit to deliver robust performance and ease of use for your investigative needs.
Product Name:
Rat MIP-1alpha (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
SKU:
AEES00472
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
