The Mouse VIPR2 (Vasoactive Intestinal Peptide Receptor 2) ELISA Kit is a powerful tool for accurately measuring VIPR2 levels in mouse samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications. VIPR2 is a key receptor involved in mediating the actions of vasoactive intestinal peptide (VIP), a neuropeptide with diverse physiological roles including neurotransmission, immune modulation, and smooth muscle relaxation.
Dysregulation of VIP and its receptor have been implicated in conditions such as inflammatory disorders, autoimmune diseases, and neurological disorders, making VIPR2 an important target for further investigation and potential therapeutic intervention. By utilizing the Mouse VIPR2 ELISA Kit, researchers can gain valuable insights into the role of this receptor in various physiological and pathological processes, advancing our understanding of VIPR2 signaling pathways and paving the way for the development of novel therapeutic strategies.
Product Name:
Mouse Vipr2 ELISA Kit
SKU:
MOFI01355
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
92-102%
83-99%
87-98%
EDTA Plasma (n = 5)
91-105%
84-93%
80-98%
Heparin Plasma (n = 5)
88-105%
87-101%
81-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-104
95
EDTA Plasma (n = 5)
87-99
94
Heparin Plasma (n = 5)
85-103
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
