In Vivo depletion atlas
In Vivo depletion atlas & Fc effector mechanisms. Functional-grade depleting antibodies remove a defined immune-cell population in vivo by flagging a surface marker for antibody-dependent killing. The top panel shows the three Fc-driven mechanisms — ADCC, CDC and ADCP; the lower panel is a pick-list of target populations. Click any marker to open the matching Assay Genie In Vivo depleting antibody, or a protein node for its ELISA kit.
A depleting antibody binds a lineage-defining surface antigen and marks that cell for destruction through its Fc region. Isotype matters: mouse IgG2a/IgG2b (and rat IgG2b) engage activating Fcγ receptors and complement most efficiently, which is why functional-grade, low / ultra-low endotoxin preparations are used for in vivo work.
Three mechanisms clear the flagged cell. In ADCC, an NK cell's FcγRIII recognises the bound antibody and releases perforin and granzyme B. In CDC, C1q docks onto clustered Fc regions and drives the complement cascade (C3 → C5b-9 membrane-attack complex). In ADCP, a macrophage's Fcγ receptor triggers phagocytosis. The net result is selective loss of the target population, letting you test its role in a model.
Assay Genie In Vivo functional-grade antibodies cover the standard depletion panel — CD4, CD8, CD3, CD90/Thy-1, CD25 (Treg), CD19 and B220 (B cells), NK1.1 (NK), Ly6G (neutrophils) and Gr-1 (myeloid/MDSC) — plus anti-CD16/CD32 for Fc-receptor blockade. For research use only; not for diagnostic or therapeutic procedures. Mouse targets shown.
Every marker links to its Assay Genie In Vivo depleting antibody; effector proteins link to the matching ELISA kit.