Anti-Phospho-GRIA1-S836 Antibody (CABP0356)

SKU:
CABP0356
€429

Description

Technical Manual

Anti-Phospho-GRIA1-S836 Antibody

Product Name:Anti-Phospho-GRIA1-S836 Antibody
Product SKU:CABP0356
Size:100 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen:A phospho specific peptide corresponding to residues surrounding S836 of human GRIA1
Sequence:

Email for sequence

Gene ID:2890
Swiss Prot:P42261
Synonyms:GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1; GluR1
Calculated MW:92kDa/93kDa/101kDa/102kDa
Observed MW:102kDa
Reactivity:Human, Mouse, Rat
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:Mouse brain
Cellular Location:Cell junction, Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, dendrite, dendritic spine, postsynaptic cell membrane, postsynaptic density, synapse

Product Images

Anti-Phospho-GRIA1-S836 Antibody (CABP0356)

Western blot analysis of extracts from mouse brain, using phospho-GRIA1-S836 antibody (CABP0356). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Uniprot Information

UniProt Protein Function:GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, calcium; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: cell junction; cell soma; cell surface; dendrite; dendritic spine; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; Golgi membrane; plasma membrane; postsynaptic density; postsynaptic membrane; recycling endosome; synaptic vesicle

Molecular Function:alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity; glutamate receptor activity; PDZ domain binding; protein binding

Biological Process: cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; ionotropic glutamate receptor signaling pathway; long-term memory; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; receptor internalization; signal transduction; synaptic transmission

NCBI Summary:Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P42261
NCBI GenInfo Identifier:116242505
NCBI Gene ID:2890
NCBI Accession:P42261.2
UniProt Secondary Accession:P42261,Q2NKM6, B7Z2S0, B7Z2W8, B7Z3F6, B7Z9G9, D3DQI4 E7ESV8,
UniProt Related Accession:P42261
Molecular Weight:102,864 Da
NCBI Full Name:Glutamate receptor 1
NCBI Synonym Full Names:glutamate ionotropic receptor AMPA type subunit 1
NCBI Official Symbol:GRIA1
NCBI Official Synonym Symbols:GLUH1; GLUR1; GLURA; GluA1; HBGR1
NCBI Protein Information:glutamate receptor 1
UniProt Protein Name:Glutamate receptor 1
UniProt Synonym Protein Names:AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic, AMPA 1; GluA1
UniProt Gene Name:GRIA1
UniProt Entry Name:GRIA1_HUMAN
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Additional Information

Product type:
Antibody
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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