The Phospho-Raf1-S259 Monoclonal Antibody (CABP1012) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly specific antibody, produced in rabbits, exhibits strong reactivity with human samples and is suitable for Western blot applications. By targeting the phosphorylated form of RAF1, this antibody enables precise detection and analysis of this important protein modification in various cell types, making it an essential tool for studies in cancer biology and signal transduction research.RAF1 is a key effector kinase in the RAS-RAF-MEK-ERK signaling pathway, which plays a central role in cell proliferation, survival, and differentiation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-Raf1-S259 Monoclonal Antibody
SKU:
CABP1012
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1557
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
STST P
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
293T treated with Forskolin, C6 treated with Forskolin
Cellular Localization:
Cell Membrane, Cytoplasm, Mitochondrion, Nucleus.
Calculated MW:
73kDa
Observed MW:
73kDa/75kDa
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method
Affinity purification
Gene ID
5894
RRID
AB_2863901
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb (CABP1012) at 1:1000 dilution. Both 293T cells and C6 cells were treated with Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
