The Phospho-SMC1-S957 Monoclonal Antibody (CABP1145) is a high-quality antibody developed for reliable detection and analysis of target proteins. SMC1 is a key component of the cohesin complex involved in regulating chromosome segregation and DNA repair. The antibody, raised in rabbits, is highly specific and sensitive for detecting phosphorylated SMC1 (S957) in human samples, making it ideal for use in Western blot applications.Phosphorylation of SMC1 at serine 957 plays a crucial role in the regulation of DNA damage response and cell cycle control. Dysregulation of this phosphorylation event has been linked to various diseases, including cancer and genetic disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-SMC1-S957 Monoclonal Antibody
SKU:
CABP1145
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1606
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
8243
RRID
AB_2864010
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit mAb (CABP1145) at 1:1000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SMC1-S957 Rabbit mAb (CABP1145) at 1:1000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Phospho-SMC1-S957 Rabbit mAb (CABP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Phospho-SMC1-S957 Rabbit mAb (CABP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-SMC1-S957 Rabbit mAb (CABP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Phospho-SMC1-S957 Rabbit mAb (CABP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
