The PNLIP Antibody (CAB6396) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to PNLIP and is validated for use in various applications, including Western blot and immunohistochemistry.PNLIP plays a crucial role in lipid metabolism, breaking down triglycerides into fatty acids and monoglycerides for absorption in the small intestine. Dysregulation of PNLIP activity has been linked to disorders such as pancreatitis and obesity, making it a target of interest in metabolic research.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PNLIP Antibody
SKU:
CAB6396
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PL, PTL, PNLIPD, PNLIP
Positive Sample:
Mouse liver, Mouse pancreas
Cellular Localization:
Secreted.
Calculated MW:
51kDa
Observed MW:
55kDa
This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea.
Purification Method
Affinity purification
Gene ID
5406
RRID
AB_2766998
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PNLIP Rabbit pAb (CAB6396) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of paraffin-embedded human pancreas using PNLIP Rabbit pAb (CAB6396) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
