The QuickStep Mouse IgG (Immunoglobulin G) ELISA Kit is a powerful tool for accurately measuring levels of mouse IgG in various biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a multitude of research applications.Mouse IgG is a key component of the immune system, functioning to protect the body against infectious agents and foreign substances. Monitoring IgG levels can provide valuable insights into immune responses, autoimmune diseases, and vaccine efficacy. The QuickStep Mouse IgG ELISA Kit is an invaluable resource for studying immune function and developing new therapeutic strategies.
Product Name:
Mouse IgG (Immunoglobulin G) Quickstep ELISA Kit
SKU:
QSES036
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
0.51 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
