The QuickStep Porcine Cortisol ELISA Kit is a cutting-edge tool specifically designed for the accurate and sensitive detection of cortisol levels in porcine samples. This kit offers high precision and reliability, ensuring consistent and reproducible results for a variety of research applications.Cortisol is a vital hormone that plays a crucial role in stress response, metabolism, and immune function in pigs. Monitoring cortisol levels in porcine samples can provide valuable insights into the health and well-being of the animals, as well as their response to various stressors and environmental conditions.
Whether studying the impact of management practices, feed additives, or environmental factors on porcine stress levels, the QuickStep Porcine Cortisol ELISA Kit is an essential tool for researchers and veterinarians alike. Trust in the accuracy and sensitivity of this kit to support your research and improve the health and welfare of your porcine subjects.
Product Name:
QuickStep Porcine Cortisol ELISA Kit
Product Code:
QSES044
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Porcine
Sensitivity:
1.41 ng/mL
Range:
3.13-200 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Cortisol
Application:
This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum and plasma.
Specificity:
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Porcine serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
9.35
24.84
70.38
9.84
25.43
72.50
Standard deviation
0.54
1.11
3.72
0.62
1.36
3.64
CV (%)
5.78
4.48
5.28
6.33
5.36
5.02
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
90-104
96
EDTA plasma(n=8)
86-98
93
Samples were spiked with high concentrations of Porcine Cortisol and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
