The Rat Cys-C (Cystatin C) ELISA Kit is a specialized assay designed for the quantitative determination of Cystatin C levels in various rat biological samples. Cystatin C, a low-molecular-weight protein, serves as a potent inhibitor of cysteine proteases and is primarily secreted by nucleated cells, with notable expression in tissues like the brain, liver, and spleen. As a biomarker of kidney function, Cystatin C has garnered significant attention for its potential in assessing glomerular filtration rate (GFR) and monitoring renal health. With its critical role in renal function, Cystatin C has been recognized as a reliable marker for kidney injury and chronic kidney disease (CKD) progression. Elevated levels of Cystatin C are associated with impaired renal function, making it a valuable indicator for diagnosing and monitoring kidney-related disorders. The quantitative measurement of Cystatin C in biological samples enables researchers to evaluate kidney function and renal health status, providing valuable insights into disease progression and treatment response. The Rat Cys-C ELISA Kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results. Manufactured under stringent quality control standards, this ELISA kit ensures robust performance and ease of use, making it a preferred tool for researchers and clinicians studying kidney diseases and related conditions.
Product Name:
Rat Cys-C (Cystatin C) ELISA Kit
SKU:
AEES00374
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
