The Rat Ferritin (FE) ELISA Kit is a specialized assay designed for the quantitative measurement and detection of ferritin levels in various biological samples obtained from rats. Ferritin is an essential intracellular protein that plays a crucial role in iron storage and release, which is vital for maintaining iron homeostasis, and acts as a biomarker for iron status and metabolism. This ELISA kit enables researchers to accurately measure ferritin concentrations, providing valuable insights into iron metabolism, storage, and utilization within the body. By offering exceptional sensitivity and specificity, this kit allows for precise and reliable quantification of ferritin levels, which is essential for understanding iron-related processes, such as iron deficiency, overload, and associated disorders. Manufactured under stringent quality control standards, this ELISA kit by Assay Genie delivers robust and reproducible results, ensuring accuracy and consistency in ferritin level measurements. With its user-friendly protocols and high performance, this kit is an excellent choice for research studies investigating iron metabolism, iron-related disorders, and the role of ferritin in maintaining overall iron homeostasis in rat models.
Product Name:
Rat FE (Ferritin) ELISA Kit
SKU:
AEES00532
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
