The NOGO Receptor 1 (RTN4R) Antibody (CAB5847) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the RTN4R protein, this antibody allows for the detection and analysis of RTN4R expression in various cell types, making it ideal for studies in neuroscience and neurodegenerative diseases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NOGO Receptor 1 (RTN4R) Antibody
SKU:
CAB5847
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NGR, NOGOR, NOGO Receptor 1 (RTN4R)
Positive Sample:
C6, Rat uterus
Cellular Localization:
Cell Membrane, Gpi-Anchor, Lipid-Anchor.
Calculated MW:
51kDa
Observed MW:
66kDa
This gene encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.
Purification Method
Affinity purification
Gene ID
65078
RRID
AB_2766597
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NOGO Receptor 1 (RTN4R) Rabbit pAb (CAB5847) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of Neuro-2a cells using NOGO Receptor 1 (RTN4R) Rabbit pAb (CAB5847) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
