10615 results for 'fluorometric assay'

Cell death is an essential aspect of cellular biology that plays a crucial role in development, homeostasis, and disease. In this blog, we try to focus on mechanisms and various type

Biochemistry · Protein Guide Amino Acids: Types, Functions, Structure & Essential Guide The 20 standard amino acids are the building blocks of

Protocols & Assay Guides | ELISA, Multiplex, Western Blot | Assay Genie

SARS-CoV-2, the causative viral agent of the novel coronavirus disease COVID-19, has sparked a global effort for creating prophylactic and therapeutic interventions. The following

Workplace Testing for COVID-19 Corporate Testing for COVID-19 The impact of COVID-19 on the econom

Oxidative Stress Definition Oxidative stress is a condition that results when the body produces or consumes more reactive oxygen species (ROS) than it can neutraliz

Metabolism · Biochemistry GuideBeta-Oxidation of Fatty AcidsBeta-oxidation is the mitochondrial pathway that breaks fatty acids down into acetyl-CoA for energy. This guide covers where it happen

Cell Culture · ProtocolsHaemocytometer Cell Counting: Procedure, Calculation and Common ErrorsA haemocytometer turns a known chamber volume into a cell concentration, and the whole method rests on fou

Disease & Pathway Tools | ELISA Kits, Antibodies & Assays by Research Area | Assay Genie

Revolutionizing Research with AI Abstract Generator Explore how GPT can help in generating scientific abstracts based on figure legends and data. Discover the potential of AI in scientific

Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful analytical biochemistry assay that utilizes antibodies and color change to identify a substance. It is widely used in the field of immunolog

Showcasing Innovation Assay Genie recently took part in an engaging event at Oxford’s BioEscalator, a leading innovation hub supporting scientific progress and entrepreneurship. The day

Quick answer On the Fatty Acid Oxidation (FAO) Assay Kit (BR00001), a researcher found that a subset of skeletal-muscle samples returned negative values once the control well was subtr

The Dynex DS2 platform fully automates the MR-proANP and MR-proADM ELISA assays, streamlining sample processing from loading to detection. Automation ensures consistent assay performance

Custom ELISA Kit Development Service | Assay Genie

Techniques · Apoptosis DetectionTUNEL Staining: The Method of Choice for Measuring Cell DeathTUNEL staining is a gold-standard technique for detecting apoptosis in situ. By labelling the fragmented DN

Free Brochures & Flyers — Product Guides | Assay Genie

Assay Genie attended AACR 2025 in ChicagoSean, Shane and David had a great few days in Chicago discussing research with scientists from across the globe at the Assay Genie booth. "It's g

Cure CLCN4 2025Assay Genie was honored to attend and present at the 2025 Cure CLCN4 Scientific Conference, held on May 19–20 at Friends House in London and online. This annual event, now

Custom In Vivo Antibody Service | Assay Genie

Custom Research Services | Assay Genie

Custom Antibody Production Service | Assay Genie

SfN 2024: Meet Assay Genie at the Society for NeuroscienceAssay Genie is delighted to be attending SfN 2024 in Chicago, Illinois on 5th to the 9th of October at the McCormick Convention

Announcement · Powered by Assay GenieIntroducing BioSymposia: The Gathering Place for ScienceWe’re excited to announce the launch of BioSymposia, our new sister platform and a free home for scientific

QuickStep ELISA Kits | 90-Minute 1-Wash Assays | Assay Genie

Metabolism · Biochemistry Guide 10 Steps of Glycolysis: Enzymes, Diagram & ATP Yield The complete ten-step pathway that converts one glucose m

Custom Protein & Peptide Service | Assay Genie

Custom Protein & Peptide Service | Assay Genie

SITC 2024: Meet Assay Genie at the Society for Immunotherapy of Cancer Annual MeetingAssay Genie is delighted to be attending and exhibiting at SITC 2024 in Houston, Texas on Friday the

AACR 2025: Meet Assay Genie at the American Association for Cancer Research Assay Genie is delighted to be attending and exhibiting at AACR 2025 in Chicago, Illinois from the 25-30th of

Animal & Veterinary ELISA Kits | Mouse, Rat, Porcine & More | Assay Genie

Contact Assay Genie | Talk to Our Scientific Team

Epidermal Growth Factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor epidermal growth factor receptor (EGFR) which triggers ligand-induced d

Department of Oncology RetreatAssay Genie was honoured to sponsor and participate in the inaugural 2025 Department of Oncology Retreat, hosted by the University of Lausanne and Lausanne

Quick answer A researcher preparing a Human IL-18 ELISA wasn't sure whether the protocol's “300-fold” HRP-Streptavidin step meant adding 40 µL of concentrat

Quick answer On the Dog Beta-endorphin (BEP) ELISA Kit, a researcher ran the same canine serum aliquots on two plates from the same lot and saw roughly double the concentration on one

IHC Antibodies | Validated Antibodies for Accurate IHC Staining | Assay Genie

Quick answer: A researcher subtracted the blank from a competitive ELISA (a Serotonin kit) and every result turned negative. In a competitive assay the signal is inversely proportional to

What is CD31? CD31, also known as PECAM-1, was originally described as a cell surface antigen present on platelets and megakaryocytes. CD31 is

As a PhD student delving deep into the realm of cellular biology, there is one antagonist that consistently challenges my research: mycoplasma. This microscopic bacterium, with its a

What is Parkinson’s disease? Parkinson's disease can be seen as a neurodegenerative disease because it is associated with the loss of nerve cel

CAR T-cell therapy CAR (Chimeric Antigen Receptor) T-cell Therapy is a type of cancer treatment. CAR T-cells are made in the laboratory and they have an artificially created receptor on the

Biologics · TNF InhibitionAdalimumab: Mechanism, Clinical Applications and BiosimilarsAdalimumab is a fully human IgG1 monoclonal antibody that binds tumour necrosis factor alpha and prevents it engag

Antibody conjugation is a pivotal technique in biomedical research and diagnostic applications. This process involves the covalent attachment of a molecule, such as a drug, toxin, enzym

Metabolism · Biochemistry Guide Cellular Respiration: Stages, ATP Production & Pathway Guide How cells convert glucose and oxygen into ATP acr

Sample Preparation · ImmunoassaySerum vs Plasma: Key Differences and When to Use EachSerum and plasma come from the same blood draw but are not interchangeable. One is collected after clotting, the ot

Localised Resources in Multiple Languages | Assay Genie

What is CD52? Cluster Of Differentiation 52 (CD52) is a highly expressed membrane glycoprotein which is present on the surface of immune cell

T cell activation is a crucial process in the immune response, playing a pivotal role in how the body responds to pathogens and maintains immune homeostasis. This intricate process involves severa

Techniques · Gene DeliveryTransduction vs Transfection: Understanding Gene Delivery TechniquesTransduction and transfection are the two main routes for introducing genetic material into cells. Transdu

Biomarkers · InflammationC-Reactive Protein (CRP): Structure, Function and Clinical UseCRP is the most widely measured marker of inflammation in medicine — fast to rise, fast to fall, and entirely non

Exploring LAG-3: A Key Regulator in T Cell ImmunityLAG-3 (Lymphocyte Activation Gene-3) is an inhibitory receptor that plays a crucial role in modulating T cell activation and effector functions. This

Free Lab Posters — Pathway Diagrams & More | Assay Genie

Time-Resolved Fluorescence (TRF) technology is a cutting-edge analytical technique that has revolutionized the fields of biochemistry, immunology, and molecular biology. By offering a refined appr

Cryopreservation of stimulated T cells is a common technique in immunology research, allowing for long-term storage while maintaining their functional viability. Stimulated T cells—especi

Cell Biology · PathologyNecrosis: Types, Causes, Mechanisms and How It Is MeasuredNecrosis is cell death accompanied by membrane rupture and release of intracellular contents — which is what makes it

QUICK ANSWER A researcher ran a competitive 2,3-bisphosphoglycerate (2,3-BPG) ELISA on red blood cell (RBC) lysate. The standard curve was textbook, yet every lysate sample read at the

What is Immunometabolism? Immunometabolism is an exponentially growing, multi-disciplinary field of research aiming at deciphering the dynamic cellular and mole

QUICK ANSWER If your ELISA returns sample concentrations that are orders of magnitude too high, the assay itself is usually fine — the error is almost always in data analysis. The most

Understanding Zonulin: Key Regulator of Intestinal Barrier Function Zonulin is a crucial physiological modulator of intercellular tight junctions, playing a significant role in regulating intestinal

Immunophenotyping by Flow Cytometry Immunophenotyping is a technique used in the field of immunology and cell biology to identify and classify cells based on the specific proteins o

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

Cell Death · BiomarkersCleaved Caspase-3: Activation, Function and Detection in ApoptosisCaspase-3 sits at the point where apoptotic signalling becomes irreversible. It is made as an inactive proenzym

Quick answer A competitive ELISA is running correctly when the blank (zero standard) gives the highest OD, the standard curve drops smoothly with rising concentration, and your samples land in the

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiplex. Sandwich ELISA is