Phenylalanine Assay Kit (Fluorometric) (BA0142)
Phenylalanine Assay Kit
L-Phenylalanine is one of the twenty common amino acids and an important precursor for several key signal molecules such as dopamine, norepinephrine, epinephrine, and the skin pigment melanin. It is found naturally in the breast milk of mammals, and used as nutritional supplements in food and drink products. The genetic disorder phenylketonuria is the inability to metabolize phenylalanine. Individuals with this disorder are known as "phenylketonurics". Individuals who cannot metabolize phenylalanine must monitor their intake of protein to control the buildup of phenylalanine.Assay Genie's L-Phenylalanine Assay Kit provides a convenient fluorimetric means to measure L-phenylalanine in biological samples. In the assay, L-phenylalanine is oxidized by phenylalanine dehydrogenase, producing NADH, which reduces a fluorescent dye to a highly fluorescent product. The resulting fluorescence intensity (Ex/Em = 530/585 nm) is linear to the L-phenylalanine concentration in the sample.
ApplicationsFor quantitative determination of L-phenylalanine and evaluation of drug effects on its metabolism.
- Safe. Non-radioactive assay.
- Sensitive and accurate. Linear detection range of 2 - 300 uM L-phenylalanine.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Phenylalanine Assay Kit Information
|Kit Includes:||Assay Buffer: 10 mL Enzyme A: 120 uL NAD Solution: 1 mL Enzyme B: 120 uL Probe: 750 uL Standard: 120 uL|
|Kit Requires:||Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader.|
|Method of Detection:||FL530/595nm|
|Detection Limit:||2 uM|
|Samples:||Serum, urine etc|
|Protocol Length:||30 min|
|Storage:||Store all reagents at -20°C.|
|Shelf Life:||6 months|