DNA Assay - Information
Assay Genie's Fluorometric DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at Ex/Em = 350/450nm, is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
For quantitative determination of DNA.
DNA Assay - Key Features
- Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay.
- Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.
- Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.
DNA Assay - Data Sheet
|Kit Includes||Reagent: 50 mL Standard: 1 mL 10 mg/mL calf thymus DNA|
|Kit Requires||Pipeting devices and accessories. 1 x TE buffer|
|Method of Detection||FL340/450nm|
|Detection Limit||100 ng/mL|
|Samples||Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices|
|Protocol Length||5 min|
|Storage||Store the DNA standard at -20°C and the Reagent at 2-8°C.|
|Shelf Life||12 months|
quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling. Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable.