The Sheep Cytokine Array (SARB0092) is a powerful research tool for studying the expression levels of 18 different cytokine targets in sheep samples. This array allows for simultaneous detection and quantification of multiple cytokines in a single experiment, providing valuable insights into immune responses and inflammatory processes in sheep.Each target on the array is carefully selected to cover a wide range of cytokine families, including interleukins, chemokines, and growth factors. By using this array, researchers can profile the cytokine expression patterns in various experimental conditions, such as infections, inflammatory diseases, and immune responses to vaccines.
The Sheep Cytokine Array is easy to use and provides reliable and reproducible results. It is suitable for a wide range of sample types, including serum, plasma, cell lysates, and tissue homogenates. With its high sensitivity and specificity, this array is an essential tool for studies in veterinary immunology, infectious diseases, and livestock health.Overall, the Sheep Cytokine Array (SARB0092) is a valuable resource for researchers interested in understanding the immune system of sheep and investigating the role of cytokines in health and disease in this important animal model.
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
AIF
Gene Symbol: N/A
Gene ID: N/A
Accession #:
Cadherin-6 (KCAD)
Gene Symbol: N/A
Gene ID: N/A
Accession #:
Decorin
Gene Symbol: DCN
Gene ID: 443048
Accession #: Q9TTE2
IFN alpha
Gene Symbol: N/A
Gene ID: N/A
Accession #:
IFN-gamma
Gene Symbol: IFNG
Gene ID: 443396
Accession #: P17773
IL-1 alpha (IL-1 F1)
Gene Symbol: IL1A
Gene ID: 443404
Accession #: Q28579
IL-1 beta (IL-1 F2)
Gene Symbol: IL1B
Gene ID: 443539
Accession #: P21621
IL-4
Gene Symbol: IL4 IL-4
Gene ID: 443327
Accession #: P30368
IL-13
Gene Symbol: IL13
Gene ID: 664707
Accession #: Q0PW91
IL-17A
Gene Symbol: IL17A
Gene ID: 101103931
Accession #: W5PWW6
IL-21
Gene Symbol: IL21
Gene ID: 100862657
Accession #: H6SGV5
LEKTI (SPINK5)
Gene Symbol: SPINK5
Gene ID: N/A
Accession #: W5P1K5
LIF
Gene Symbol: LIF
Gene ID: N/A
Accession #: W5P8L4
MIG (CXCL9)
Gene Symbol: N/A
Gene ID: N/A
Accession #:
RANTES (CCL5)
Gene Symbol: CCL5
Gene ID: N/A
Accession #: W5P4G7
sFRP-3
Gene Symbol: FRZB
Gene ID: 101118419
Accession #: W5Q5L6
TNF alpha
Gene Symbol: TNF, TNFA, TNFSF2
Gene ID: 443540
Accession #: P23383
VEGF-A
Gene Symbol: VEGFA, VEGF
Gene ID: 443103
Accession #: P50412
Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex
Sheep Cytokine Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.
Block membranes
2.
Incubate with Sample
3.
Incubate with Biotinylated Detection Antibody Cocktail
4.
Incubate with HRP-Conjugated Streptavidin
5.
Incubate with Detection Buffers
6.
Image with chemiluminescent imaging system
7.
Perform densitometry and analysis
Chauffert, B., et al.
Protracted Intraventricular Infusion of Gemcitabine to Circumvent the Blood Brain Barrier