The Thioredoxin 1 (Trx1/TXN) Monoclonal Antibody (CAB4024) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Thioredoxin 1 (Trx1/TXN) Monoclonal Antibody
SKU:
CAB4024
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2658
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:2000 - 1:10000
IHC-P
1:1000 - 1:8000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TRX, TRDX, TRX1, Trx80, Thioredoxin 1 (Trx1/TXN)
Positive Sample:
HeLa, MCF7, C2C12, Mouse lung, Rat brain
Cellular Localization:
Cytoplasm, Nucleus, Secreted.
Calculated MW:
12 kDa
Observed MW:
12 kDa
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7295
RRID
AB_2863174
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Thioredoxin 1 (Trx1/TXN) Rabbit mAb (CAB4024) at 1:10000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Thioredoxin 1 (Trx1/TXN) Rabbit mAb (CAB4024) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using Thioredoxin 1 (Trx1/TXN) Rabbit mAb (CAB4024) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
