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Anti-Tubulin alpha 1b Antibody (CABC024)

SKU:
CABC024
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC024
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC024
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC024
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC024
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC024
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Description

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Antibody Name:Anti-Tubulin alpha 1b Antibody
Antibody SKU:CABC024
Antibody Size:100 uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-451 of human Tubulin alpha 1b (NP_006073.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, PC-12, A-549, U-251MG, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-451 of human Tubulin alpha 1b (NP_006073.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MREC ISIH VGQA GVQI GNAC WELY CLEH GIQP DGQM PSDK TIGG GDDS FNTF FSET GAGK HVPR AVFV DLEP TVID EVRT GTYR QLFH PEQL ITGK EDAA NNYA RGHY TIGK EIID LVLD RIRK LADQ CTGL QGFL VFHS FGGG TGSG FTSL LMER LSVD YGKK SKLE FSIY PAPQ VSTA VVEP YNSI LTTH TTLE HSDC AFMV DNEA IYDI CRRN LDIE RPTY TNLN RLIS QIVS SITA SLRF DGAL NVDL TEFQ TNLV PYPR IHFP LATY APVI SAEK AYHE QLSV AEIT NACF EPAN QMVK CDPR HGKY MACC LLYR GDVV PKDV NAAI ATIK TKRS IQFV DWCP TGFK VGIN YQPP TVVP GGDL AKVQ RAVC MLSN TTAI AEAW ARLD HKFD LMYA KRAF VHWY VGEG MEEG EFSE ARED MAAL EKDY EEVG VDSV EGEG EEEG EEY
Gene ID:10376
Uniprot:P68363
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:37kDa/50kDa
Observed MW:52kDa
Anti-Tubulin alpha 1b Antibody (CABC024)Western blot analysis of extracts of various cell lines, using Tubulin alpha 1b antibody (CABC024) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Tubulin alpha 1b Antibody (CABC024)Immunohistochemistry of paraffin-embedded Mouse spleen using Tubulin alpha 1b antibody (CABC024) at dilution of 1:50 (40x lens).
Anti-Tubulin alpha 1b Antibody (CABC024)Immunohistochemistry of paraffin-embedded Rat kidney using Tubulin alpha 1b antibody (CABC024) at dilution of 1:50 (40x lens).
Anti-Tubulin alpha 1b Antibody (CABC024)Immunofluorescence analysis of NIH/3T3 cells using Tubulin alpha 1b antibody (CABC024) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-Tubulin alpha 1b Antibody (CABC024)Immunofluorescence analysis of C6 cells using Tubulin alpha 1b antibody (CABC024) at dilution of 1:100. Blue: DAPI for nuclear staining.
Synonyms:TUBA1B, K-ALPHA-1
Background:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
UniProt Protein Function:TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; cytoplasmic microtubule

Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process

UniProt Code:P68363
NCBI GenInfo Identifier:55977474
NCBI Gene ID:10376
NCBI Accession:P68363.1
UniProt Secondary Accession:P68363,P68366,
UniProt Related Accession:P68363
Molecular Weight:55kDa
NCBI Full Name:Tubulin alpha-1B chain
NCBI Synonym Full Names:tubulin alpha 1b
NCBI Official Symbol:TUBA1B  
NCBI Official Synonym Symbols:K-ALPHA-1  
NCBI Protein Information:tubulin alpha-1B chain
UniProt Protein Name:Tubulin alpha-1B chain
UniProt Synonym Protein Names:Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Protein Family:Tubulin
UniProt Gene Name:TUBA1B  
UniProt Entry Name:TBA1B_HUMAN

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