The ANKHD1 Antibody (CAB8224) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been rigorously validated for use in Western blot applications. By binding to the ANKHD1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in cell biology and cancer research.ANKHD1 is known to play a crucial role in cell proliferation and differentiation, making it a key target for investigations into cancer development and progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ANKHD1 Antibody
SKU:
CAB8224
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MASK, MASK1, VBARP, PP2500, ANKHD1
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
269kDa
Observed MW:
269kDa
This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments.
Purification Method
Affinity purification
Gene ID
54882
RRID
AB_2768365
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ANKHD1 Rabbit pAb (CAB8224) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
