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Protein Glycosylation for Labs: Mechanisms, Workflows, and Reagent Tips Protein glycosylation is the enzymatic covalent attachment of sugar chains (glycans) to specific amino acid side chains, most commonly asparagine (N-linked) or serine and threonine (O-linked). It is the most common and structurally diverse post-translational and co-translational modification in eukaryotic cells, and it governs whether a nascent polypeptide folds correctly, survives ER quality control, reaches the cell surface, and gets recognized by the immune system or a binding partner. Table of Contents What Is Protein Glycosylation and What Types Exist? How Does N-Linked Glycosylation Work Mechanistically? How …