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RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein from cultured cells. RIPA buffer cell lysis enables determination of protein concentration. RIPA buffer is an ideal cell lysis reagent since it contains three non-ionic and ionic detergents. Preserving protein:protein interactions When you need to preserve protein …

The meaning is the name Flow = in motion Cyto = cells Metry = measure. Flow cytometry measures the properties of cells while in a fluid stream. It enables single cell analysis of complex cellular systems (e.g. blood) very rapidly (100s of cells/second) it also allows you to look at various cellular properties such as size, granularity, fluorescence intensity per cell. Flow Cytometry can be used for cell counting, cell sorting, biomarker detection and protein engineering. Cell component …

Introduction The process of transformation allows foreign DNA to be introduced into a cell. Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bacterial origin and contain both a bacterial origin of replication and an antibiotic resistance gene which can be used as a selectable marker.Genetic modifications can be made to various bacterial …

Introduction Sonication applies sound energy to agitate particles in your sample. The ultrasonic frequency used is usually greater than >20 kHz. In an experimental setting this is usually carried out using an ultrasonic bath or ultrasonic probe generally referred to as sonication. In the laboratory sonication is used mainly as a method of cell disruption. Sonication is used to disrupt cellular membranes and release the cells contents, this process is ge …

Our ELISA protocol provides a step by step guide to preparing uncoated ELISA plates, washing ELISA plates and analyzing your ELISA data. In this step by step ELISA assay protocol guide we provide detailed information on preparing your microtitre plates by coating them with primary antibody, blocking your plates with BSA and capture antibody and lysing your samples and setting up your ELISA plates. Our infographic also gives an overview of ELISA assay plate set up and measuring your ELI …