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Validate Western Blot Antibodies: Datasheet Checks Labs Should Ask Reliable results come from antibodies validated specifically for denatured western blot conditions: recombinant monoclonals or well-characterized monoclonals for high specificity, polyclonals when you need sensitivity for low-abundance or mutated targets. Confirm every candidate with knockout or orthogonal validation, match your secondary antibody’s host and conjugate to your detection system, and never assume a clean single band proves specificity on its own. Table of Contents How Do You Choose the Right Primary Antibody for Western Blot? Which Secondary Antibody Matches Your Primary and Detection Goals? What Should You Ch …