Canine IL-1 Ra/IL-1 F3 ELISA Kit

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Canine IL-1 Ra/IL-1 F3 ELISA Kit Data

Product SKU: SBRS2024
Size: 96T
Application: This ELISA antibody pair recognizes Canine IL-1ra.
Uniprot: Q9BEH0
Gene ID: 403660
Gene Names: IL1RN
Synonyms: Interleukin-1 receptor antagonist protein (IL-1RN) (IL-1ra) (IRAP) (IL1 inhibitor)
Target Species: Canine
Compatible Sample Types: Cell Culture Supernatants, Plasma, Serum
Design Principle: Sandwich-based
Method of Detection: Colorimetric
Quantitative/Semi-Quantitative: Quantitative
Range: 29.5 ng/ml - 8000 pg/ml
Sensitivity: 29.5 pg/ml
Recommended Dilution: 5 fold
Storage/Stability: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Kit Components:

1. Pre-Coated 96-well Strip Microplate
2. Wash Buffer
3. Stop Solution
4. Assay Diluent(s)
5. Lyophilized Standard
6. Biotinylated Detection Antibody
7. Streptavidin-Conjugated HRP
8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Canine IL-1ra ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 ul of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 ul of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 ul of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 ul of Stop Solution to each well.
11. Read at 450 nm immediately.