Caspase (Cysteine-requiring Aspartate Protease) is a protease family that plays an important role in the process of apoptosis. Caspase 8 (also known as FLICE, MACH or mch5) is considered to be a caspase in the upstream of apoptotic signal transduction. Caspase 8 is activated in Fas-receptor and TNFR-1-mediated apoptotic process, forming a dimer composed of P18 and p10, which further activates downstream Caspase 4, Caspase 6, Caspase 9 and Caspase 10. Caspase 8 exists in the form of prozyme in the normal state and has no activity. However, Caspase 8 is activated in the apoptotic stage and participates in the apoptotic process.\nThis kit is used to conjugate Caspase 8 sequence-specific peptides acetyl-Ile-Glu-Val-Asp p-nitroanilide (Ac-IETD-pNA) to yellow group p-nitroaniline (pNA). When the substrate is cut by Caspase 8, the yellow group pNA is dissociated. pNA has an absorption peak at 405 nm. Measure the OD value at 405 nm and then Caspase 8 activity can be calculated accordingly
Overview Background One step rapid dewaxing solution is a rapid reagent for paraffin slides pretreatment. There is no need to do the traditional three times of xylene dewaxing, three to five...
Overview Background 7-AAD Reagent (100µg/mL) is developed to identify apoptotic and necrotic cells. 7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded...
Overview Background Cell Staining Buffer, a buffer optimized for sample preparation of flow cytometry (FCM), can be used in fluorescent antibody dilution and cell sample washes. This product...
Overview Background 10xRBC Lysis/Fixation Solution is designed for FCM sample processing of human whole blood. The whole blood is treated with anticoagulant and incubated with fluorescent...
