CEP85 Rabbit Polyclonal Antibody (CAB15891)

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CAB15891
  • Western blot analysis of extracts of various cell lines, using CEP85 antibody (CAB15891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
  • Immunofluorescence analysis of C6 cells using CEP85 antibody (CAB15891) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using CEP85 antibody (CAB15891) at dilution of 1:100. Blue: DAPI for nuclear staining.
€99 - €249

Description

Technical Manual

CEP85 Rabbit Polyclonal Antibody

Product Name:CEP85 Rabbit Polyclonal Antibody
Product SKU:CAB15891
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a protein that belongs to the centrosome-associated family of proteins. The centrosome is a subcellular organelle in the animal cell that functions as a microtubule organizing center and is involved in cell-cycle progression. Alternate splicing results in multiple transcript variants.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 130-430 of human CEP85 (NP_073615.2).
Sequence:

TRNGDLGAMKHSPGLSRDLMYFSGATGENGIEQSWFPAVGHERQEEARKFDIPSMESTLNQSAMMETLYSDPHHRVRFHNPRTSTSKELYRVLPEAKKAPGSGAVFERNGPHSNSSGVLPLGLQPAPGLSKPLPSQVWQPSPDTWHPREQSCELSTCRQQLELIRLQMEQMQLQNGAICHHPAAFGPSLPILEPAQWISILNSNEHLLKEKELLIDKQRKHISQLEQKVRESELQVHSALLGRPAPFGDVCLLRLQELQRENTFLRAQFAQKTEALSREKIDLEKKLSASEVEVQLIRESL

Gene ID:64793
Swiss Prot:Q6P2H3
Synonyms:CEP85; CCDC21
Calculated MW:22kDa/80kDa/85kDa
Observed MW:105kDa
Reactivity:Human, Mouse, Rat
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunofluorescence 
Positive Samples:A-431, Jurkat, HeLa,
Cellular Location:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus, spindle pole

Product Images

CEP85 Rabbit Polyclonal Antibody (CAB15891)

Western blot analysis of extracts of various cell lines, using CEP85 antibody (CAB15891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

CEP85 Rabbit Polyclonal Antibody (CAB15891)

Immunofluorescence analysis of C6 cells using CEP85 antibody (CAB15891) at dilution of 1:100. Blue: DAPI for nuclear staining.

CEP85 Rabbit Polyclonal Antibody (CAB15891)

Immunofluorescence analysis of HeLa cells using CEP85 antibody (CAB15891) at dilution of 1:100. Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:CCDC21: Belongs to the CEP85 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: Golgi apparatus; spindle pole; centrosome; cytoplasm; nucleolus

Molecular Function:protein binding

NCBI Summary:This gene encodes a protein that belongs to the centrosome-associated family of proteins. The centrosome is a subcellular organelle in the animal cell that functions as a microtubule organizing center and is involved in cell-cycle progression. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:Q6P2H3
NCBI GenInfo Identifier:528078329
NCBI Gene ID:64793
NCBI Accession:NP_001268446.1
UniProt Secondary Accession:Q6P2H3,Q5VY68, Q5VY70, Q9H6Q1, Q9H828, Q9UF52, B4DRL1 D3DPK4, F8W7K4,
UniProt Related Accession:Q6P2H3
Molecular Weight:80,302 Da
NCBI Full Name:centrosomal protein of 85 kDa isoform 2
NCBI Synonym Full Names:centrosomal protein 85kDa
NCBI Official Symbol:CEP85 
NCBI Official Synonym Symbols:CCDC21 
NCBI Protein Information:centrosomal protein of 85 kDa
UniProt Protein Name:Centrosomal protein of 85 kDa
UniProt Synonym Protein Names:Coiled-coil domain-containing protein 21
Protein Family:Centrosomal protein
UniProt Gene Name:CEP85 
UniProt Entry Name:CEP85_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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