Human D betaH (Dopamine- beta-Hydroxylase) CLIA Kit
The Human D-BetaH Dopamine Beta Hydroxylase CLIA Kit is a reliable and highly sensitive assay designed for the accurate detection of D-BetaH levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring dependable results for a variety of research applications.D-BetaH is an important enzyme involved in the synthesis of dopamine, playing a crucial role in the nervous system. Dysregulation of D-BetaH activity has been linked to various neurological disorders, such as Parkinson's disease and schizophrenia. Therefore, accurate measurement of D-BetaH levels is essential for understanding the pathophysiology of these conditions and developing potential therapeutic interventions.Overall, the Human D-BetaH Dopamine Beta Hydroxylase CLIA Kit is a valuable tool for researchers studying neurobiology and related disorders, providing reliable data for further exploration and discovery.
Product Name:
Human D betaH (Dopamine- beta-Hydroxylase) CLIA Kit
SKU:
HUES00699
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.