Human Leucine Rich Repeat LGI Family, Member 3 (LGI3) ELISA Kit
The Human Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit is specifically designed for the precise measurement of LGI3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.LGI3 is a protein that belongs to the LGI family and is known to play a critical role in various cellular functions, including cell adhesion and neuronal development. Abnormal levels of LGI3 have been associated with certain neurological disorders and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially developing new treatment strategies.
With its advanced technology and reliability, the Human LGI3 ELISA Kit is a valuable tool for researchers looking to explore the role of LGI3 in health and disease. This kit provides a convenient and efficient method for detecting LGI3 levels, enabling researchers to gain valuable insights into the role of this protein in various physiological and pathological processes.
Product Name:
Human Leucine Rich Repeat LGI Family, Member 3 (LGI3) ELISA Kit
Product Code:
HUDL01712
Size:
96 Assays
Target:
LGI3
Synonyms:
LGIL4, LGI1-like protein 4, Leucine-rich glioma-inactivated protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
