Human PGF2 alpha (Prostaglandin F2 alpha) ELISA Kit
The Human PGF2-alpha (Prostaglandin F2-alpha) ELISA Kit is specially designed for the accurate measurement of PGF2-alpha levels in human samples like serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reproducible results that are crucial for various research applications.PGF2-alpha is a key prostaglandin involved in various physiological processes such as smooth muscle contraction, inflammation, and reproduction. It plays a vital role in conditions like asthma, inflammation, and reproductive disorders, making it a valuable biomarker for understanding these diseases and exploring potential therapeutic interventions. With the Human PGF2-alpha ELISA Kit, researchers can confidently quantify PGF2-alpha levels in human samples, leading to a deeper understanding of the role of this bioactive lipid and its implications in different disease states. Trust this kit for accurate and reliable results that can drive groundbreaking research in biomedical and pharmaceutical fields.
Product Name:
Human PGF2 alpha (Prostaglandin F2 alpha) ELISA Kit
SKU:
HUES02795
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
