The Rat PGF2-alpha (Prostaglandin F2-alpha) ELISA Kit is specifically designed for accurate measurement of PGF2-alpha levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results, making it ideal for a variety of research applications.PGF2-alpha is a potent prostaglandin known for its role in inflammation, smooth muscle contraction, and reproductive processes. Its levels are often elevated in conditions such as asthma, hypertension, and uterine disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions. With this ELISA kit, researchers can easily quantify PGF2-alpha levels in rat samples, leading to a better understanding of its role in physiology and pathology. Whether investigating inflammatory responses, reproductive health, or cardiovascular function, this kit provides a reliable and efficient tool for studying PGF2-alpha in rat models.
Product Name:
Rat PGF2 alpha (Prostaglandin F2 Alpha) ELISA Kit
SKU:
RTES00662
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
