Human PIIINP (N-Terminal Procollagen 3 Propeptide) CLIA Kit
The Human PIIINP (N-terminal propeptide of type III procollagen) CLIA Kit is a reliable and accurate assay for the quantitative measurement of PIIINP levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing researchers with dependable and reproducible results for a variety of research applications.PIIINP is a key biomarker associated with collagen synthesis and tissue remodeling, making it a valuable tool for studying conditions such as fibrosis, liver disease, and bone metabolism. Understanding PIIINP levels can provide insight into disease progression and treatment outcomes, making this CLIA kit an essential component in biomarker research and therapeutic development.
Product Name:
Human PIIINP (N-Terminal Procollagen 3 Propeptide) CLIA Kit
SKU:
HUES00157
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
