Human PIIINP (N-Terminal Procollagen 3 Propeptide) ELISA Kit
The Human PIIINP (N-terminal procollagen 3 propeptide) ELISA Kit is specifically designed for the quantitative measurement of PIIINP levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.PIIINP is a key biomarker for collagen synthesis and remodeling, providing valuable insights into conditions such as fibrosis, liver disease, and bone disorders. By detecting and measuring PIIINP levels, researchers can gain a deeper understanding of these conditions and potentially identify new therapeutic targets.Whether studying disease mechanisms, monitoring treatment efficacy, or conducting basic research, the Human PIIINP ELISA Kit offers a powerful tool for investigating collagen metabolism and related pathologies. Trust in its precision and performance to advance your research goals.
Product Name:
Human PIIINP (N-Terminal Procollagen 3 Propeptide) ELISA Kit
SKU:
HUES01450
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
14.06 pg/mL
Detection range:
23.44-1500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
