Human TDG Recombinant Protein (RPPB2308)
- SKU:
- RPPB2308
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13569
- Research Area:
- Enzymes
Description
| Product Name: | Human TDG Recombinant Protein |
| Product Code: | RPPB2308 |
| Size: | 20µg |
| Species: | Human |
| Target: | TDG |
| Synonyms: | Thymine-DNA Glycosylase, G/T Mismatch-Specific Thymine DNA Glycosylase, EC 3.2.2.29. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TDG solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA |
Thymine-DNA glycosylase (TDG) is a member of the TDG/mug DNA glycosylase family. TDG is a nuclear protein that fixes G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. In addition, TDG removes uracil and 5-bromouracil from mispairings with guanine. The TDG enzyme has an essential role in cellular defense against genetic mutation triggered by the spontaneous deamination of 5-methylcytosine and cytosine.
TDG Human Recombinant produced in E. coli is a single polypeptide chain containing 433 amino acids (1-410) and having a molecular mass of 48.4 kDa.TDG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family. |
| UniProt Protein Details: | Protein type:DNA repair, damage; EC 3.2.2.29; Nuclear receptor co-regulator; Hydrolase Chromosomal Location of Human Ortholog: 12q24.1 Cellular Component: nucleoplasm; PML body; plasma membrane; nucleus Molecular Function:protein domain specific binding; protein binding; protein homodimerization activity; structure-specific DNA binding; SUMO binding; protein kinase C binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; transcription factor binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; mismatched DNA binding Biological Process: transcription from RNA polymerase II promoter; embryonic development; mismatch repair; base-excision repair, AP site formation; depyrimidination; base-excision repair; gene expression; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; DNA repair; negative regulation of protein binding; regulation of gene expression, epigenetic |
| NCBI Summary: | The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13569 |
| NCBI GenInfo Identifier: | 46397791 |
| NCBI Gene ID: | 6996 |
| NCBI Accession: | Q13569.2 |
| UniProt Secondary Accession: | Q13569,Q8IUZ6, Q8IZM3, |
| UniProt Related Accession: | Q13569 |
| Molecular Weight: | 410 |
| NCBI Full Name: | G/T mismatch-specific thymine DNA glycosylase |
| NCBI Synonym Full Names: | thymine-DNA glycosylase |
| NCBI Official Symbol: | TDG�� |
| NCBI Official Synonym Symbols: | hTDG�� |
| NCBI Protein Information: | G/T mismatch-specific thymine DNA glycosylase |
| UniProt Protein Name: | G/T mismatch-specific thymine DNA glycosylase |
| UniProt Synonym Protein Names: | Thymine-DNA glycosylase; hTDG |
| Protein Family: | G/T mismatch-specific thymine DNA glycosylase |
| UniProt Gene Name: | TDG�� |
| UniProt Entry Name: | TDG_HUMAN |
