Human VCAM-1/CD106 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
The Human VCAM-1 (CD106) ELISA Kit is specially designed for the precise measurement of Vascular Cell Adhesion Molecule-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research purposes.Vascular Cell Adhesion Molecule-1 (VCAM-1) is a key molecule involved in cellular adhesion and inflammation, playing a crucial role in various diseases such as atherosclerosis, inflammation, and autoimmune disorders.
By detecting VCAM-1 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Human VCAM-1 (CD106) ELISA Kit is a valuable tool for studying the role of VCAM-1 in disease mechanisms and developing novel treatment strategies.
Product Name:
Human VCAM-1/CD106 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
SKU:
HUES03559
Target:
Human VCAM-1/CD106 (Vascuolar Cell Adhesion Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAM-1/CD106. You can calculate the concentration of Human VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
98-109
90-107
Average (%)
100
103
98
1:4
Range (%)
91-102
85-99
82-97
Average (%)
97
91
89
1:8
Range (%)
90-105
82-93
88-99
Average (%)
96
87
93
1:16
Range (%)
92-105
82-95
81-94
Average (%)
99
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-98
90
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.82
11.41
47.65
5.08
11.96
47.72
Standard deviation
0.27
0.5
2.48
0.28
0.5
2.07
C V (%)
5.6
4.38
5.2
5.51
4.18
4.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Human VCAM-1/CD106 and analogues was observed.
