The [KO Validated] DNMT1 Monoclonal Antibody (CAB19679) is a high-quality antibody developed for reliable detection and analysis of target proteins. DNMT1 is essential for maintaining DNA methylation patterns during cell division, and dysregulation of this enzyme has been implicated in numerous diseases, including cancer and neurological disorders.This antibody, specifically raised in rabbits, has been rigorously tested and validated for knockout (KO) studies, ensuring high specificity and sensitivity in detecting DNMT1 protein levels. It is suitable for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, allowing for detailed analysis of DNMT1 expression and localization in different cell types and experimental conditions.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] DNMT1 Monoclonal Antibody
SKU:
CAB19679
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC51348
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1786
RRID
AB_2862730
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KD Validated] DNMT1 Rabbit mAb (CAB19679) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 30 s.
Western blot analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells, using [KD Validated] DNMT1 Rabbit mAb (CAB19679) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
