Description
system_update_altDatasheet
Mouse Cytokine Array (62 targets) (SARB0081)
The Mouse Cytokine Array (SARB0081) is a powerful tool for researchers studying cytokine expression in mouse samples. This array kit allows for the simultaneous detection of 62 different cytokines, providing a comprehensive view of the immune response in a single experiment.With its high sensitivity and specificity, this cytokine array enables accurate quantification of cytokine levels in various biological samples, including serum, plasma, cell culture supernatants, and tissue lysates. The array is easy to use, making it suitable for both experienced researchers and those new to cytokine analysis.
By utilizing the Mouse Cytokine Array, researchers can gain valuable insights into the molecular mechanisms underlying immune responses, inflammation, and disease pathology. This array kit is an essential tool for studies in immunology, cancer research, and other fields where cytokine profiling is essential for understanding disease processes and developing new therapeutic strategies.
| Product Sku: | SARB0081 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 62 |
| Gene Symbols: | AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL24, CCL25|CCL27, CCL3, CCL5, CCL9, CD40, CSF1, CSF2, CSF2RB, CSF3, CX3CL1, CXCL1, CXCL10, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL9, FASLG, IFNG, IGFBP3, IGFBP5, IGFBP6, IL10, IL12A, IL13, IL17A, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, LEPR, PF4, SELL, SELP, THPO, TIMP1, TNF, TNFRSF8, TNFSF8, TPO, VCAM1, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Axl
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
| CD40 (TNFRSF5)
|
CRG-2
| CTACK (CCL27)
| CXCL16
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Fas Ligand (TNFSF6)
| Fractalkine (CX3CL1)
| GCSF
| GM-CSF
| IFN-gamma
|
IGFBP-3
| IGFBP-5
| IGFBP-6
| IL-1 beta (IL-1 F2)
| IL-10
|
IL-12 p40/p70
| IL-12 p70
| IL-13
| IL-17A
| IL-1 alpha (IL-1 F1)
|
IL-2
| IL-3
| IL-3 R beta
| IL-4
| IL-5
|
IL-6
| IL-9
| KC (CXCL1)
| Leptin
| Leptin R
|
LIX
| L-Selectin (CD62L)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-5
|
M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
|
MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
| Platelet Factor 4 (CXCL4)
| P-Selectin
| RANTES (CCL5)
|
SCF
| SDF-1 alpha (CXCL12 alpha)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TARC (CCL17)
|
I-309 (TCA-3/CCL1)
| TECK (CCL25)
| TIMP-1
| TNF alpha
| Thrombopoietin (TPO)
|
VCAM-1 (CD106)
| VEGF-A
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
