NADP/NADPH Assay Kit
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable.Assay Genie's NADP/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the NADP/NADPH concentration in the sample. This assay is highly specific for NADP/NADPH and with minimal interference (<1%) by NAD/NADH. Our assay is a convenient method to measure NADP+, NADPH and their ratio.
ApplicationsFor quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.
- Sensitive and accurate. Detection limit of 0.01 uM and linearity up to 1 uM NADP+/NADPH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
NADP/NADPH Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL Enzyme A: 120 mL Glucose (1 M): 1.5 mL Enzyme B: 120 mL Probe: 750 mL NADP Standard: 0.5 mL NADP/NADPH Extraction Buffers: each 12 mL|
|Kit Requires:||Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at FL »ex/em = 530/585 nm.|
|Method of Detection:||FL530/585nm|
|Detection Limit:||0.01 uM|
|Samples:||Cell, tissue extracts etc|
|Protocol Length:||30 min|
|Storage:||Store all reagents at -20°C|
|Shelf Life:||6 months|