NADP/NADPH Assay Kit (Fluorometric) (BA0107)

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SKU:
BA0107
$903.13

Description

ELISA Kit Technical ManualMSDS

NADP/NADPH Assay Kit

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable.

Assay Genie's NADP/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the NADP/NADPH concentration in the sample. This assay is highly specific for NADP/NADPH and with minimal interference (<1%) by NAD/NADH. Our assay is a convenient method to measure NADP+, NADPH and their ratio.

Applications

For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

Key Features

  • Sensitive and accurate. Detection limit of 0.01 uM and linearity up to 1 uM NADP+/NADPH in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

NADP/NADPH Assay Kit Information:

Kit Includes: Assay Buffer: 10 mL Enzyme A: 120 mL Glucose (1 M): 1.5 mL Enzyme B: 120 mL Probe: 750 mL NADP Standard: 0.5 mL NADP/NADPH Extraction Buffers: each 12 mL
Kit Requires: Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at FL »ex/em = 530/585 nm.
Method of Detection: FL530/585nm
Detection Limit: 0.01 uM
Samples: Cell, tissue extracts etc
Species: All
Protocol Length: 30 min
Size: 100 tests
Storage: Store all reagents at -20°C
Shelf Life: 6 months

 

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