Description
Rat Chemokine Array (15 targets) (SARB0100)
The Rat Chemokine Array (SARB0100) is a comprehensive research tool designed to analyze the expression levels of 15 different chemokines in rat samples. These chemokines play a crucial role in immune response regulation, inflammation, and cell migration. The array allows for simultaneous detection and quantification of multiple chemokines in a single experiment, providing researchers with valuable information about the immune system's response to various stimuli. The high sensitivity and specificity of the array ensure accurate results, making it an essential tool for studies in immunology, inflammation, and disease pathology.
With the ability to analyze multiple chemokines at once, researchers can gain a comprehensive understanding of the intricate immune response mechanisms in rats, shedding light on potential targets for therapeutic interventions. The Rat Chemokine Array is a valuable asset for any research project aiming to unravel the complexities of immune regulation and inflammatory processes in rat models.
| Product Sku: | SARB0100 |
| Size: | 2, 4, or 8 |
| Species Detected: | Rat |
| Number of Targets Detected: | 15 |
| Gene Symbols: | CCL11, CCL2, CCL20, CCL27, CCL3, CCL4, CX3CL1, CXCL1, CXCL2, CXCL3|CXCL5, CXCL9, IL1A, PPBP, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
MIP-3 alpha (CCL20)
| CINC-1 (CXCL1)
| CINC-2
| CINC-3
| CTACK (CCL27)
|
Eotaxin-1 (CCL11)
| Fractalkine (CX3CL1)
| IL-1 alpha (IL-1 F1)
| LIX
| MCP-1 (CCL2)
|
MIG (CXCL9)
| MIP-1 beta (CCL4)
| MIP-1 alpha (CCL3)
| TCK-1 (CXCL7)
| TNF alpha
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Rat Chemokine Array 1
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
