CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. CD40LG is a member of the TNF superfamily and, as with other family members, exists as a trimer in membrane-bound form and a soluble form that is bioactive. CD40L is a ligand for CD40; in cells expressing CD40L, ligation of this protein activates signal transduction. CD40LG is a T-cell antigen that is expressed on the surface of the cell. It promotes B-cell growth and production of all immunoglobulins isotypes in the presence of cytokines. CD40LG has been found to cause cytokine production and tumour-killing activity in peripheral blood monocytes, according to a study. It also stimulates the proliferation of activated T-cells, as well as IFN-gamma production, and TNF-alpha; and IL2. Human CD40L/TNFSF5 Recombinant Protein (E.coli) is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human CD40L/TNFSF5 Recombinant Protein (E.coli) (RPES3661)
Immobilized Human CD40L at 5µg/ml(100 ?l/well) can bind Human CD40-Fc(Cat: PKSH032216). The ED50 of Human CD40L is 68.55ug/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human CD40L is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
16.2 kDa
AP Mol Mass:
15 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 200mM NaCl; 0.1mM EDTA; pH 7.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
